Part:BBa_K5056012
Modified CM-biomimetic peptide- linker - cell-penetrating peptide
C-rds is the peptide modified from original C. militaris biomimetic peptide (BBa_K5056005) by having the first valine and isoleucine replaced by phenylalanine and leucine, respectively. By examining ACPred and ENNACT - two distinct databases developed by other researchers - C-rds shows high score in both database against lung cancer.
Two restriction sites (NdeI and SacI) have been added in both ends for digestion and ligation.
6X His tag has been added at the beginning for purification.
One restriction site, NheI, has been added between 6X His tag and the C. militaris biomimetic peptide for future modification.
A middle linker is added between C. militaris biomimetic peptides and cell-penetrating peptide
Polyarginine Cell-Penetrating Peptide: Peptides, especially intracellular functional peptides that can play a particular role inside a cell, have attracted attention as promising materials to control cell fate. However, hydrophilic materials like peptides are difficult for cells to internalize18. Octaarginine (R8) as a Polyarginine Cell-Penetrating Peptide (CPP) is reported to enhance peptide’s ability to cross the cell membrane and deliver cargo19. Hence, ‘RRRRRRRR’ is used as our signal peptide sequence for penetrating cell membrane and enter the cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 61
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 128
- 1000COMPATIBLE WITH RFC[1000]
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